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Fig. S1
Notch signaling reporter lines based on the TP1bglob module. (A) Tg(Tp1:H2BmCherry) drives expression of H2BmCherry, which has a very long half-life, in NRCs. Tg(Tp1:VenusPest) drives expression of a destabilized VenusPEST fluorescent protein, which has a short half-life, in NRCs. (A-C) Tg(Tp1:H2BmCherry); Tg(Tp1:VenusPest) larvae were treated with DMSO or DAPT from 4 to 6 dpf. (B) DMSO-treated larvae are Tg(Tp1:H2BmCherry)+ and Tg(Tp1:VenusPest)+. (C) DAPT-treated larvae are Tg(Tp1:H2BmCherry)+ but strongly downregulate or even lose Tg(Tp1:VenusPest) expression in most tissues. Arrowheads indicate the IPD. (D,D2) An individual Tg(Tp1:GFP) larva was imaged at 3.5 dpf, incubated in DAPT until 5.5 dpf and imaged again. (E,E2) An individual Tg(Tp1:VenusPest) larva was imaged at 3.5 dpf, incubated in DAPT until 5.5 dpf and imaged again. Tg(Tp1:VenusPest) fluorescence is strongly downregulated compared with Tg(Tp1:GFP) fluorescence in D. Arrowheads indicate the IPD. (F) Embryos were obtained from an in-cross of mibta52b heterozygous parents. Wild-type Tg(Tp1:GFP) embryos at 2.5 dpf show strong Tg(Tp1:GFP) expression in multiple organs, whereas mib homozygous mutant embryos, recognized by their curly tail phenotype, failed to express Tg(Tp1:GFP). (G) Tg(Tp1:H2BmCherry) larvae were fixed at 7 dpf and stained for 2F11 (blue) to mark IPD cells (white arrowheads). The Tg(Tp1:H2BmCherry)+ cells (red) in the pancreas correspond to the 2F11+ IPD cells. 2F11+ and Tg(Tp1:H2BmCherry)+ cells ventral to the IPD are gut cells. All images are lateral views, anterior towards the right, ventral towards the bottom, except in F, where anterior is towards the top and ventral towards the right.