ZFIN Image: Veleri et al., 2012, Fig. 3

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Figure Caption

Fig. 3

Human mRNA rescues zebrafish bbs9-spMO phenotype.

hW and hM represent wild type and mutant human mRNA, respectively. The arrows indicate eye phenotype. (A) The uninjected control (top) and bbs9-spMO alone injected (bottom) zebrafish at 72 hpf. (B) Rescue of bbs9-spMO eye phenotype by hW 100 pg (top), but not by lower dose of 50 pg (bottom). (C) The bbs9-spMO phenotype is not rescued by hM as the eye defect remains in the morphants. (D) The quantification of embryos′ eye size at 72 hpf in rescue experiment using human mRNAs co-injected with bbs9-spMO. X-axis shows category of embryos scored. Y-axis shows the eye size in pixels. Data are presented as mean ± SEM. Statistically significant and non-significant observations are indicated with p value and n.s., respectively.

Acknowledgments

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