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Fig. S10
Cell death in the spinal cords of gle1 morphants is suppressed by expressing human GLE1. (A,B) gle1 morphants injected with buffer alone (A) or with <140 pg of in vitro transcribed EGFP-tagged human GLE1b mRNA (B) were co-stained with anti-Islet1/2 and anti-cleaved caspase 3 antibodies at 34 hpf. Note that the cleaved caspase 3 signals were diminished when human GLE1 was expressed in the gle1 morphant (B). (C,D) gle1 morphants with their motoneurons labeled by mnx1:TagRFP-T transgene were injected with buffer alone (C) or with <140 pg of in vitro transcribed EGFP-tagged human GLE1b mRNA (D). These morphants were stained with Acridine Orange to reveal dying cells and the live embryos were imaged at 50 hpf. Note that apoptotic cell death was suppressed by expressing human GLE1 (D). Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bars: 50 μm.