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Fig. S4 Injection of 75 pg wobble arl6ip1 mRNA enables embryos to rescue the defects induced by 4 ng arl6ip1-MO1. (A-C) Dorsal views of 3-somite-stage embryos, anterior to the top. (D-F) Lateral views of 24 hpf embryos, anterior to the left. (A, B) Compared to wild-type embryos, decreased expression of foxd3 was evident and predominant, especially in caudal region of pre-migratory neural crest cells in arl6ip1 morphants. (C) The down-regulation of foxd3 in arl6ip1-MO1 embryos was rescued by the wobble arl6ip1 mRNA. (D, E) Crestin-positive neural crest cells that normally migrate ventrally from the neural tube into the trunk were disrupted in arl6ip1 morphants (indicated by arrows). (F) Co-injection of wobble arl6ip1 mRNA with arl6ip1-MO1 recovered neural crest migration. (G) Quantification of the number of streams of sox10-labeled neural crest cells certified that migratory defects in arl6ip1 morphants can be rescued by wobble arl6ip1 mRNA.