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Fig. 6
Effects of MO-mediated zfp29 knockdown in Zebrafish.
One hundred zebrafish embryos at 2-cell stage were injected with control and zfp29MO. 76 embryos had phenotypic defects. (A) Lateral view of live embryos at 24 hpf. (B) Embryos were injected with control and zfp29 MO and then incubated with acridine orange or incubated with anti-acetylated tubulin antibody at 24 hpf. Increased apoptotic cells were shown with acridic orange (AO) in zfp29 depleted cells. Reduction of acetylated α-tubulin and less motor neurites were identified in zfp29 morphants.
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