ZFIN Image: Kirchmaier et al., 2012, Fig. 5

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Figure Caption

Fig. 5 Murine Popdc2 is able to rescue the morphant phenotype. (A–H) Lateral view of the (A,E) uninjected wild-type embryos (WT) as controls and embryos injected with either (B,F) 100 pg Popdc2 cRNA, (C,G) 1 ng of MO1-popdc2, or (D,H) 100 pg Popdc2 cRNA and MO1-popdc2 morpholino, which were photographed at (A,C,E,G) 24 hpf, or (B,D,F,H) 48 hpf. (I–L) Lateral confocal stacks of the zebrafish trunk labeled with phalloidin rhodamine at 48 hpf. (I) control, (J) Popdc2 cRNA, (K) MO1-popdc2, (L) Popdc2 cRNA and MO1-popdc2 morpholino. (M) Graphic depiction of the percentage of embryos with wildtype appearance (WT) (blue), or weak (pink), and severe (red) pathological phenotypes. Significant difference (p < 0.05) is marked with an asterisk. Scale bar = 100 μm.

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.