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Fig. 4 Distribution of BrdU-labeled cells in the injured adult zebrafish telencephalon. (A) Adult zebrafish were placed in water containing 10 mM BrdU for 4 hours, and then in BrdU-free water. After creating the lesion, they were then sacrificed at the indicated time points. (B–E′) Immunodetection of BrdU in the coronal brain sections (dorsal up) at 0 (B,B′), 3 (C,C′), 7 (D,D′) and 10 (E,E′) dpl. Higher magnifications of the boxed areas in panels BE are shown in B′–E′, respectively. Arrows show injury-induced accumulations of BrdU-positive cells. White dotted circles indicate the injury site. VZ, ventricular zone; Sub, subpallium; Pa, pallium; mPa, medial pallium; dPa, dorsal pallium; lPa, lateral pallium. Scale bars: 100 ¼m. (F–H) Histograms showing the counts of BrdU-positive cells in the telencephalic VZ (F), in the subpallium (Sub) and pallium (Pa) (G), and in the medial-dorsal-lateral domain of the telencephalic pallium (mdlPa) (H). Student’s t-test was used to determine significant differences in expression. Error bars represent s.e.m. *P<0.05, **P<0.01, ***P<0.001.