ZFIN Image: Padanad et al., 2012, Fig. 10

Image

Figure Caption

Fig. 10

Misexpression of Fgf or Pax8 cannot expand otic development without Foxi1. (A–F) Expression of pax8 at 13 hpf. (G–L) Expression of pax2a at 13 hpf. Embryos were heat shocked at 10 hpf, and most embryos were injected at the one-cell stage with foxi1-MO (B–F, H–L) as indicated across the top of the figure. Genotypes of transgenic embryos, including hs:fgf8/hs:fgf8 (C, I), hs:fgf3/hs:fgf3 (D, J), hs:pax8/+ (E, K), and hs:fgf8/+; hs:pax8/+ (F, L) are indicated across the top of the figure. Positions of midbrain–hindbrain boundary (mhb), pronerphros (pn) and otic placode (op) are indicated. Note that the pronerphric domain of pax2a is also expanded anteriorly in double transgenic embryos (L). All images show dorsal views with anterior to the top. Scale bar, 150 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 364(1), Padanad, M.S., Bhat, N., Guo, B., and Riley, B.B., Conditions that influence the response to Fgf during otic placode induction, 1-10, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.