Fig. 2

Fig. 2

Stage-dependent effects of Fgf misexpression. (A–F) Embryos were heat shocked at 7 hpf and fixed at 10.5 hpf to examine expression of pax8 in the otic primordium (A–C) or 30 hpf to examine expression of cldna in the otic vesicle (D–F). (G–L) Embryos were heat shocked at 10 hpf and fixed at 13 hpf to examine otic expression of pax2a (G–I) or at 30 hpf to examine expression of cldna (J–L). Genotypes of wild-type controls and heterozygous transgenic embryos are indicated across the top of the figure. Images show lateral views with anterior to the left (A–F, J–L); dorsal views with anterior to the top (G–I). Scale bar, 50 μm. (M) Summary of the effects of activating hs:fgf8 at various developmental stages.