ZFIN Image: Liang et al., 2012, Fig. 7

Image

Figure Caption

Fig. 7 RA inhibits primitive myelopoiesis by acting downstream of gata4/5/6.

All embryos are positioned anterior left and lateral front. Embryos were microinjected with gata5-MO and gata6-MO together (B, C, G, H) at 1–2-cell stage and then treated with 10 μM DEAB (C, H) or vehicle DMSO (B, G) immediately, or microinjected with gata4 mRNA and gata6 mRNA together (D, E, I, J) and then treated with 250 nM RA (E, J) or vehicle DMSO (D, I) from 10 to 11 hpf, respectively. They were then examined for expressions of myeloid markers lcp1 (A–E) and mpx (F–J) at 24 hpf by whole mount in situ hybridization. The number shown in the lower left-hand corner of each panel is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed.

Acknowledgments

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