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Fig. 4
Determination of cancer cell dissemination kinetics.
(A and B) LnCAP (A) or PC3 cells (B) were implanted and embryos were fixed at 2, 4, or 6 dpi for imaging (immunofluorescence images and automated image analysis (scatter plots)). Bottom row images (scale bar = 50 μm) show zoom-in of area marked by dotted line in top row images (scale bar = 100 μm). (C) CD at 2, 4 and 6 dpi for LnCAP (grey) and PC3-injected embryos (black) calculated from scatterplots in A and B, respectively. Statistical testing for difference between LnCAP and PC3 at different dpi is indicated. *p<0.05, ***p<0.001.
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