ZFIN Image: Nicoli et al., 2012, Fig. S2

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Figure Caption

Fig. S2

miR-221 is not required for arteriovenous differentiation.

(A) Whole mount in situ hybridization of embryos injected with 10 ng of control (left) or miR-221 (right) morpholino. Embryos were fixed at 28 hpf and subjected to whole mount in situ hybridization using DIG-labeled antisense riboprobes against the indicated transcripts. Lateral views, dorsal is up, anterior to the left. Dorsal aorta and posterior cardinal vein are indicated by a bracket and A or V respectively. ISVs are indicated by arrows in the control panel for kdrl. Scale bar is 50 μm.

(B) qRT-PCR quantification of dll4 and flt4 expression at 28 hpf in embryos injected with 10 ng miR-221. MO. Levels are relative to embryos injected with 10 ng control Morpholino.

(C) RT-PCR for flt4 and ef1alpha in 28 hpf embryos injected with indicated Morpholinos.

(D) Relative levels of miR-221 in embryos at 28 hpf injected with indicated Morpholinos compared to control embryos.

Acknowledgments

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Reprinted from Developmental Cell, 22(2), Nicoli, S., Knyphausen, C.P., Zhu, L.J., Lakshmanan, A., and Lawson, N.D., miR-221 Is Required for Endothelial Tip Cell Behaviors during Vascular Development, 418-429, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell