ZFIN Image: Warp et al., 2012, Fig. S4

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Figure Caption

Fig. S4

Gap Junction Blocker Heptanol Has Little Effect on Substantial Fraction of Cells in Younger Embryos, But Profoundly Blocks Activity in Older Embryos, Related to Figure 4

GCaMP3 population activity before and after heptanol treatment in an 18 (A) and 20.5 (B) hpf embryo.

(1) Baseline GCaMP3 fluorescence images with cells active in pre-heptanol movies circled.

(2) GCaMP3 intensity traces (ΔF/F) for these regions before (left) and after (right) 2mM heptanol treatment shows that some cells remained spontaneously active during gap junction block in the younger embryo (A2; cells 2 and 4), but no cells were active after heptanol treatment in the older embryo (B2).

Acknowledgments

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