ZFIN Image: Martin et al., 2012, Fig. S2

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Figure Caption

Fig. S2

Newly created HS:TCFΔC and HS:caβcat transgenic lines have expected downstream effects on embryonic development, related to Figures 2 and 6

In order to confirm the specificity of the HS:TCFΔC transgenic line, side by side heat-shocks were done with the HS:dkk1 transgenic line and analyzed for gross morphology or gene expression. The HS:TCFΔC embryonic phenotype is exactly the same as HS:dkk embryos (e and f compared to c and d). (g) In order to confirm that the newly created transgenic lines are affecting the expression of downstream Wnt target genes, the expression of several known direct targets of canonical Wnt signaling was examined. Embryos were heat-shocked at dome stage and fixed at 75% epiboly for tbx6, sp5l, and ntl expression (Martin and Kimelman, 2008; Szeto and Kimelman, 2004; Weidinger et al., 2005). In all cases, there was a strong increase in expression of target genes in HS:caβcat embryos, and a strong decrease in expression in HS:TCFΔC embryos.

Acknowledgments

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Reprinted from Developmental Cell, 22(1), Martin, B.L., and Kimelman, D., Canonical Wnt Signaling Dynamically Controls Multiple Stem Cell Fate Decisions during Vertebrate Body Formation, 223-232, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell