ZFIN Image: Li et al., 2011, Fig. 10

Image

Figure Caption

Fig. 10 Knockdown of endogenous Smyd1b advances the timing of sarcomeric localization of Smyd1b_tv1^myc in zebrafish embryos.

A. Smyd1b E9I9-MO was injected into Smyd1b_tv1^myc or Smyd1b_tv1^myc transgenic zebrafish embryos at 1–2 cell stages. Western blot analysis shows the expression of myc-tagged Smyd1b_tv1^myc and Smyd1b_tv1^myc in un-injected control or E9I9-MO injected transgenic zebrafish embryos at 24 hpf. γ-Tubulin was used as loading control. B and C. Immunostaining using anti-myc antibody shows the cytoplasmic (B) or sarcomeric localization (C) of smyd1b_tv1^myc in control (B) or E9I9-MO injected (C) transgenic zebrafish embryos at 24 hpf. D and E. Immunostaining using anti-myc antibody shows the cytoplasmic localization of smyd1b_tv2^myc in control or E9I9-MO injected transgenic zebrafish embryos at 24 hpf. Scale bar: 30 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One