ZFIN Image: Li et al., 2011, Fig. 8

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Figure Caption

Fig. 8 The Serine 225 is required for the enhanced sarcomeric localization of Smyd1b_tv1.

A. Sequence comparison shows that the alternative splicing of smyd1b in various vertebrates and the conserved serine and threonine residues within the 13 aa insertion. B and C. Immunostaining using anti-myc antibody shows the sarcomeric localization of Smyd1b_tv1^myc in myofibers of zebrafish embryos at 38 hpf. C represents the highlighted box area in A. D–G. Immunostaining using anti-myc antibody shows the sarcomeric localization of Smyd1b_tv1^myc mutant proteins that carry substitutions at S217A and T221A (D), S225A (E), S225T (F), S225D (G). Scale bars: B = 40 μm; C = 20 μm.

Acknowledgments

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