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Fig. S2 Generation, characterisation and effects of a grhl2b exon 1/intron 1 splice-blocking morpholino. (A) Schematic of PCR primers used to detect non-spliced reverse-transcribed mRNA product following block of splicing of grhl2b. (B) A specific grhl2b non-spliced product was detectable by RT-PCR; this product was absent in MO:control-injected fish. (C,D) The grhl2b splice-blocking MO reproduced the MHB-folding (C) and apoptosis (TUNEL; D) phenotype observed with the grhl2b ATG-blocking MO. The apoptosis in grhl2b morphants is most abundant in, but not restricted to, neural regions.
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