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Fig. S4 FGF signaling regulates foxj1a expression and cilia length and number in a dose-dependent manner. (A-C) foxj1a expression was downregulated in DFCs in embryos incubated with 10 μm of SU5402 (B) and further reduced in embryos incubated with 20 μm of SU5402 (C). Shown are dorsal views of 90% epiboly staged embryos. (D-F) DFC-targeted injection of fgfr1 MO inhibited foxj1a expression dose dependently. (G-I) DFC-targeted injection of fgfr1 MO did not affect DFC specification as revealed by normal sox17 expression. (J,K) Quantification of KV cilia length and number. SU5402 was incubated with embryos at 60% epiboly stage for 20 minutes. *P<0.01. Approximately 12-20 embryos at 10 somites were examined for each group. Error bars represent s.d.