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Fig. 6 Elevated cell death in itga5 morphants is rescued by hs:fgf8.
<>(A–D) Transgenic pax2a:GFP embryos immunostained for GFP (green) and Caspase3 (red). Images show dorsal views (anterior up) of the right otic/epibranchial domain in a control embryo (A), itga5 morphant (B), hs:fgf8/+ embryo (C) and hs:fgf8/+ embryo injected with itga5-MO (D). White arrows mark apoptotic cells. All embryos were heat shocked at 39°C for 30 minutes beginning at 11.5hpf and fixed at 13.5 hpf. Scale bar, 50 μm. (E) Mean number of Caspase3-positive cells in the otic/epibranchial domain in each of the four groups of embryos. Error bars indicate S.E.M.
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