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Fig. S3
Loss of Smad1/5/8 phosphorylation in heat shocked Tg(hs:dnBmpr1a-GFP) embryos. (A-F) Immunochemical staining for pSmad1/5/8, lateral views, anterior towards the left. (A-C) GFP-negative, non-transgenic controls subjected to heatshock. (D-F) GFP-positive transgenics. (A,D) pSmad1/5/8 staining is already reduced in arches 1 and 2 in transgenics 2 hours post-heatshock (post-hs). (B,E) Staining is lost in the arches and in the eye by 4 hours post-hs. (C,F) 6 hours post-hs. 1, mandibular arch; 2, hyoid arch; de, dorsal eye.
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