|
Fig. 7
Edn1 rescues ventral patterning in BMP-deficient embryos. (A-O) Whole-mount in situ hybridization to detect expression of genes involved in DV arch patterning at 30 hpf, lateral views, anterior towards the left in controls (A,D,G,J,M), Tg(hsp70I:dnBmpr1a-GFP) transgenics heat shocked at 16-18 hpf (B,E,H,K,N) and heat-shocked transgenics injected with human recombinant EDN1 protein (C,F,I,L,O). hand2 (A-C) expression is unaffected, whereas dlx5a (G-I), dlx6a (J-L) and msxe (M-O) expression are restored by EDN1 protein in heat-shocked transgenics, and dlx3b (D-F) expression is induced throughout the DV extent of the arches. Arches 1 and 2 are numbered. Arrows indicate rescued gene expression. Scale bar: 100 μm.