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Fig. S2 DNA injection of PCNA-GFP does not affect cell cycle length or phase proportions. (A) DNA injection of PCNA-GFP construct labels retinal nuclei mosaically and, as with RNA injection, all phases of the cell cycle are readily identifiable (arrowheads). (B) Cell cycle phase lengths are broadly similar to those seen with RNA injection. Error bars indicate s.d. and the number of nuclei measured are indicated in brackets. (C,D) Confocal slices through 56 hpf retinas transgenically expressing red fluorophores under the control of ptf1a (dsRed) and ath5 (RFP) promoters. The retinal lamination of an uninjected embryo (C) is compared with a PCNA-GFP-injected embryo (D) and no difference is seen. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: 10 μm.