ZFIN Image: Kroehne et al., 2011, Fig. S10

Image

Figure Caption

Fig. S10 her4.1 is expressed by radial glia showing reactive proliferation after stab lesion. These cells can be lineage traced using tg(her4.1:mcherryT2ACreERT2) transgenic fish. (A) her4.1 is expressed along the whole extent of the ventricular proliferation zone of the telencephalon, as shown by in situ hybridisation. (B) her4.1-expressing radial glia in the VZ react to lesion and re-enter the cell cycle. After stab lesion, tg(her4.1:EGFP) transgenic fish many nuclei of EGFP⁺ (GFP) cells (green) co-localize with PCNA (red, arrows) 3 dpl, as shown in single confocal sections (high magnification inset). Note that EGFP expression in this line highly mimics radial glia morphology (radial processes, arrowheads). Scale bars: 200 μm in A; 100 μm in B, 10 μm in inset (high magnification). Dashed outlines represent the lesion.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development