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Fig. 7 foxc1a function is required for the initiation of endocardial development but not for inhibition of myocardial development. (A-H) hand2 (A-D) and cmlc2 (E-H) expression is not affected in Foxc1a morphant embryos, as analyzed by in situ hybridization at the 10-somite (A-D) and 20-somite (E-H) stages. (I-L) nfatc1 expression (arrows) is reduced moderately at low foxc1a MO doses of 1.5 ng (J) and severely at higher doses of 3 ng and 4 ng (K,L) as detected by in situ hybridization. (A-L) Flat-mounted ventral views, anterior is upwards. (M) The severity and number of morphant embryos exhibiting endocardial reduction of nfatc1 increases with the dose of foxc1a morpholino, while myocardial hand2 and cmlc2 expression is not affected significantly.