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Fig. 1
XF24 islet plate setup.
Four wells were used as temperature control wells (wells A1, B3, C4 and D6) and as such contained no embryos. Embryos were plated in all other wells in numbers that allowed for respiration and media acidification measurements to be made within the recommended range. Inset: Expanded view of a 30 hpf zebrafish embryo in an islet plate well prior to assay.
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