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Fig. S8 Specificity of Sfrp1a morpholinos. (A) Position of antisense morpholinos designed to block Sfrp1a translation on the sequence of 52-UTR and the 52 part of sfrp1a ORF. Injection of 180 pg of Sfrp1a-GFP mRNA at the one-cell stage results in embryos expressing the GFP at the late blastula stage (B). Coinjection of Sfrp1a-GFP mRNA with 8 ng of morpholino Sfrp1a_1 (C) or of Sfrp1a_2 (D) prevents translation of the Sfrp1a-GFP fusion protein. (Left) Bright field. (Right) Visualization of GFP fluorescence.
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