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Fig. S1 Effects of a short term exposure to retinoic acid on retinoic acid signaling and rod photoreceptors. Embryos carrying the RARE-YFP transgene were treated with either DMSO (A and C) or 0.3 μM RA (B and D) from 36 to 39 hpf. (A and B) Embryos were processed at 49 hpf as 4 μm cryosections for anti-GFP indirect immunofluoresence. (A) Control embryos exhibiting endogenous transgene expression in the ventral retina. (B) Embryos treated with the ′pulse′ of RA show strong ventral labeling, as well as very weak labeling elsewhere in the retina. (C and D) Embryos were processed at 60 hpf for whole mount in situ hybridization with a probe directed against rod opsin mRNA. (C) Control embryos show the normal distribution of rods across the retina. (D) Embryos treated with the RA ′pulse′ show a similar distribution and density of rod photoreceptors. Bar = 50 μm.