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Fig. S5

ID
ZDB-IMAGE-110920-53
Source
Figures for Bohnsack et al., 2011
Image
Figure Caption

Fig. S5

T3 and T4 partially restore T4 expression in PTU-treated embryos. Wholemount immunostaining for T4 in 96 hpf embryos (ventral view) demonstrated that in the absence of PTU exogenous treatment with 100 nM T3 and 100 nM T4 between 6 and 96 hpf mildly decreased T4 expression in thyroid follicles (C) compared to embryos treated with T3 and T4 from 6 to 22 hpf (B) and untreated controls (A). Treatment with 0.003% (D) and 0.03% PTU (G) progressively decreased T4 expression compared to embryos raised in the absence of PTU (A). Treatment with exogenous T3 and T4 from 6 to 22 hpf (E) or from 6 to 96 hpf (F) did not improve the effect of 0.003% PTU on T4 expression (D) while exogenous T3 and T4 from 6 to 96 hpf (I), but not from 6 to 22 hpf (H) slightly improved T4 expression in embryos treated with 0.03% PTU (G).

Acknowledgments
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