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Fig. S2
Effect of PTU is time sensitive. 72 hpf Tg(α-actin::EGFP) embryos treated with PTU at 22 hpf and 10 μM DEAB (A, B) at 24 hpf showed shortening of the jaw and only 2 pharyngeal arches, but minimal effect on extraocular muscle development compared to 0.1% DMSO (C, D). In the roy background and in the absence of PTU, treatment with 15 μM DEAB at 24 hpf (E, F) or 10 μM DEAB at 18 hpf (G, H) also disrupted jaw and pharyngeal arch formation, but did not cause thickening or loss of extraocular muscles.
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