Fig. 1

Fig. 1

Overexpression of Pou5f1-VP16 can ventralize wild-type embryos while overexpression of Pou5f1-En can dorsalize. (A) Live photographs of a wild-type (WT) zebrafish embryo at 33–36 h post fertilization (28.5 °C) (top) and of embryos that were injected with pou5f1-En mRNA (20 pg; D1–D4) or pou5f1-VP16 mRNA (40 pg; V1–V4) at the one-cell stage within 30 min of egg laying. As an injection control, membrane-GFP mRNA (30 pg) was co-injected, and embryos with low GFP expression at early shield stage were removed. Lateral views, anterior to left, dorsal at top. Dorsoventral index modified from Kishimoto et al. (1997). (B) Dorsoventral index of WT zebrafish embryos scored at 33–36 hpf. The graph shows percent of phenotypes (V1–V4, D1–D4 as in (A), dead and normal) from total number of injected embryos. (C) Whole-mount in situ hybridization of pooled WT embryos injected with pou5f1-VP16 mRNA (30 or 50 pg) or pou5f1-En mRNA (40 or 110 pg) at the one-cell stage, and of non-injected WT control embryos, showing expression of chordin at 70% epiboly.