ZFIN ID: ZDB-IMAGE-110812-44
Figures for Lim et al., 2011

Figure Caption/Comments:

Fig. S4

Distinguishing between transplanted muscle pioneers and transplanted endothelial cells. (A) Heterochronic transplant scheme used for this test: donor is labeled with Rhodamine Dextran (RhDx) and both donor and host are marked with fli1a:egfpy1. (B-D) Live lateral views of a 48 hpf transplanted embryo. There are three classes of RhDx-positive cells at the HMS. (1) A RhDx+ GFP- cell. This is a transplanted muscle pioneer (MP, white arrow). (2) A RhDx+ GFP+ cell in which the GFP and RhDx fluorescence coincide. This is a transplanted endothelial cell (EC, yellow arrow). (3) A RhDx+ cell adjacent to, but not coinciding with, a GFP+ cell. This is a transplanted MP adjacent to a host EC (blue arrow). For rescue experiments in which the host was not marked with fli1a:egfpy1, class 3 combines with class 1; however, it is still possible to distinguish between transplanted MPs and transplanted ECs by looking for coincident GFP expression from the donor fli1a:egfpy1 transgene.

Figure Data:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.