ZFIN ID: ZDB-IMAGE-110812-36
Figures for Lim et al., 2011

Figure Caption/Comments:

Fig. 3

Transplanting wild-type MPs at the HMS rescues PAC formation in netrin 1a morphants. (A) Scheme for heterochronic transplants. Rhodamine Dextran (RhDx) is injected into one-cell stage fli1a:egfpy1 zebrafish embryos (donor). netrin1aSBMO is injected into one-cell stage fli1a:egfpy1 embryos (host). Cells are transferred from the RhDx embryo at 4 hpf to the netrin 1a morphant host at 3 hpf at a location fated to include MPs. (B-G) Lateral views showing fli1a:egfpy1 and the lineage marker (RhDx) in two transplanted embryos. The PAC (arrows, B,E) forms in the same hemisegments that receive transplanted wild-type MPs (arrowheads, C,F). Confocal projections. (H) Quantitation of rescue in netrin 1a morphants showing presence of the PAC (partial or complete) in hemisegments that either received wild-type transplanted MPs or were populated only by morphant host MPs. Host MPs only, 42±9%; transplanted donor MPs, 78±10%. All values are mean±s.e.m.; P-value determined by Wilcoxon signed rank test. n, number of hemisegments (number of embryos). A, anterior; D, dorsal; MP, muscle pioneer. Scale bar: 50 μm.

Figure Data:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.