ZFIN Image: Garkavtsev et al., 2011, Fig. 5

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Fig. 5

DAL interferes with adhesive properties of endothelial cells in vitro. (A) DAL is involved in actin cytoskeleton assembly regulation. For F-actin visualization, we plated HUVECs and treated them with saline or DAL (10 μM) for 3 h. After fixing and permeabilizing these cultured cells, we subsequently stained them with phalloidin. (B) DAL interferes with adherens junctions in networks of HUVEC cells cultured on matrigel. After 24 h, control HUVEC cells organized into a network of cordlike structures, whereas DAL-treated HUVECs show disjoined networks. Existing cordlike structures, when exposed to 10 μM of DAL for 3 h, were also disrupted. (C) A wound-healing motility assay with HUVECs. We disrupted HUVEC monolayers mechanically with a pipette tip and followed the migration of cells to “heal” the wound using microscopy. DAL (5 μM) slowed healing compared with the control, shown at 24 h after wounding.

Acknowledgments

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