ZFIN Image: Murphy et al., 2011, Fig. 1

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Figure Caption

Fig. 1

Tks5 is required for embryonic development in Danio rerio.

(A) Schematic of Tks5 targeted morpholinos (MO) and the zebrafish Tks5 gene. (B) The morphology of Tks5 morphants (T5.1 MO+p53 MO) at 48 hpf compared to controls (Uninjected, Control MO, p53 MO). Enlarged images of the tail region show a reduction in posterior pigment cells (dashes outline tail in morphants). Scale bar represents 200 μm. (C) Tks5 MO specificity was determined by injecting embryos with either tks5:GFP mRNA or tks5:GFP mRNA together with Tks5 MO, and analyzing GFP expression. Two representative embryos from each injection are shown. (D) Quantification of murine Tks5 rescue of Tks5 morphant phenotypes. Embryos were injected as indicated in Experimental Procedures. The total number of morphants (white bars) was compared to the total number of normal embryos (black bars), and quantified as described in Materials and Methods (n = 3).

Figure Data

Phenotype details

Acknowledgments

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