ZFIN Image: Rieger et al., 2011, Fig. S5

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Figure Caption

Fig. S5

Knockdown of duox1 blocks the growth-promoting effects of amputation in p53 morphant larvae. (A) RT-PCR showing knockdown of duox1 wildtype transcript after morpholino injection as in [13]. Arrows point to the relevant bands. (B–D) Time-lapse sequences from 78–90 hpf. The rightmost panel shows axon tip trajectories (red) over the course of the time-lapse; denervated territories are indicated by shaded areas. (B) Enhanced axon growth in a p53 control-MO-injected larval fin after amputation (dotted line) and reinnervation of denervated territory (shaded area). (C) Co-injection of p53-MO and duox1-MO prevented axon growth and reinnervation after amputation. (D) Rescue of axon growth inhibition and reinnervation in p53-MO/duox1-MO double morphants in the presence of 1.5 mM H₂O₂. See quantification in Figure 6F.

Acknowledgments

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