ZFIN Image: Ikenaga et al., 2011, Fig. s6

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Figure Caption

Fig. s6

(Magenta/Green version of Figure 9 for the assistance of color blind readers.) Spinal neurons in pax2a/pax8 double mutant. A, B: RFP fluorescence of a pax8+/m embryo (A) and a double mutant embryo (B) under the same optical condition. The RFP signal is reduced in the double mutant. Scale: 1 mm. C, D: The RFP signal in the spinal cord of a pax8+/m embryo (C) and a double mutant embryo (D) near the 15th body segment. 3D images were constructed from confocal slices. Scale: 20 μm. E-H: Stochastic labeling by GFP (green) identified a neuron with an ipsilateral descending axon in the double mutant. E is a 3D reconstruction. F (RFP), G (GFP), and H (merged) are single plane images. Scale: 50 μm. I, J: The spinal cord of a pax8+/m larva obtained from crossing with the Alx-GFP transgenic line. The image is from an embryo at 4 dpf. I is a 3D reconstruction and J is a single confocal plane. Note that RFP and GFP do not overlap. Scale: 20 μm.

Acknowledgments

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