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Fig. S3
Validation of the fgf10a splice MO. Embryos were injected with 4 ng of the fgf10a MO, which targets the boundary of the 1st exon and 1st intron, harvested at 28 hpf, and used for RT-PCR analysis. fgf10a cDNA including the 2nd exon and part of the 1st and 3rd exons was amplified from control but not fgf10a MO-injected embryos, indicating that the MO blocks the proper splicing of fgf10a transcript. Elongation factor 1a cDNA was amplified from both control and fgf10a MO-injected embryos.
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