ZFIN Image: Durán et al., 2011, Fig. 2

Image

Figure Caption

Fig. 2

Colocalization of Collagen type I and Type II in actinotrichia during development and regeneration. Immunofluorescence against Collagen I (red) and Collagen II (green). Sequential confocal sections were obtained in the Z-axis of the whole thick of the samples. Projections of the Z-stack are shown for the red (A), the green (B) and the merged channels (C) of the fin fold of a doubly immunostained 72 hpf embryo. A confocal plane of the merged red and green channels, at a higher magnification, shows the actinotrichia fibers in detail (D). Z-stack projections of all confocal sections .of a 4 dpa longitudinal fin section doubly immunostained and with nuclei counterstained in blue (E–G). E: Red and blue channels. F: Green and blue channels. G: All-merged channels. H: A detail of all merged channels shows colocalization of the green and red labels. Bars represent 100 μm in A–C and E–G; and 25 μm in D and H. Arrowhead is actinotrichia. e is epidermis. b is basal epidermal layer. c is connective tissue. n is notochord.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 354(1), Durán, I., Marí-Beffa, M., Santamaría, J.A., Becerra, J., and Santos-Ruiz, L., Actinotrichia collagens and their role in fin formation, 160-172, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.