ZFIN Image: Bai et al., 2011, Fig. 2

Image

Figure Caption

Fig. 2

Zebrafish P0 is a 23.5 kDa glycoprotein. A: A western blot probed with P0 antibody. Whole adult zebrafish brain lysate (32 μg protein per lane) was incubated with (+; right lane) or without (+ left lane) PNGaseF, prior to SDS-PAGE and western transfer. B: A short segment of the predicted extracellular domain of P0 is shown is shown (amino acids are numbered with respect to the full open reading frame). The consensus N-glycosylation motif is shown in bold. Underlining denotes the asparagine residue to which the N-acetyl-glucosamine side chain is linked. C: The N terminus of P0 is shown, illustrating the predicted signal peptide cleavage sites. D: The table shows the size and predicted molecular mass of P0 after cleavage of the signal peptide at each of the putative cleavage sites.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.