ZFIN Image: Wilkinson et al., 2009, Fig. S8

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Figure Caption

Fig. S8 Induction of tBR Line by Heat Shock at 21hpf Does Not Affect The Polarisation of tbx20 to the Dorsal Wall of the DA.

a-h) Expression of tbx20 (red and green arrowheads) from 23-26hpf in embryos heat shocked at 21hpf. Whereas tbx20 expression within the dorsal aorta (green arrowheads) is unaffected in GFP- (black letters) or GFP+ embryos (green letters), GFP+ embryos demonstrate a loss of ventral expression under the yolk cell extension (red arrowheads), as observed previously (Pyati et al., 2006). i, j) Transgenic embryos heat shocked at 21hpf were subjected to in situ hybridisation for tbx20, sectioned to a thickness of 10μm and counterstained with nuclear fast red. Exclusion of tbx20 from the ventral wall of the DA was observed in both GFP- (i) and GFP+ embryos (j) at 24hpf (green arrows). Abbreviations: NC, notochord.

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Reprinted from Developmental Cell, 16(6), Wilkinson, R.N., Pouget, C., Gering, M., Russell, A.J., Davies, S.G., Kimelman, D., and Patient, R., Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta, 909-916, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell