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Fig. 6
Chromatin immunoprecipitation using both Dmrt1bY::GFP and deltaDmrt1bY::GFP transgenic lines respectively expressing either Dmrt1bY or a control truncated Dmrt1bY (delta DM form lacking the DNA binding domain) fused to GFP revealed specific in vivo Dmrt1bY protein affinity to the Izanagi-nested dmrt1 target site, including the one described within dmrt1bY promoter. (A) Transgenic lines established for in vivo tissue–ChIP. (B) Dmrt1bY promoter primer sets map. (C) Specific enrichment of Izanagi-nested Dmrt1 binding sites subsequent to in vivo Dmrt1bY immunoprecipitation.
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