ZFIN Image: Robles et al., 2011, Fig. 3

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Figure Caption

Fig. 3

The dlx5/6:GFP transgene labels a subpopulation of glutamatergic neurons. (A) Confocal image of vglut2b fluorescence in situ hybridization in a 5-dpf dlx5/6:GFP larvae. (B) 2× magnification of regions indicated by boxes in (A). Merged images of GFP immuofluorescence and vglut2b in situ labeling (left) and vglut2b in situ labeling alone (right). GFP fluorescence was used to trace cell body outlines. Note strong, punctate vglut2b in situ signal in the perinuclear region of cell body in B2. In contrast, the majority of cells in the SPV (B1) and all cells in the superficial NP (B3) lacked this punctate vglut2b mRNA signal. (C) Quantification of glutamatergic phenotypic analysis in dlx5/6:GFP. Scale bar, 20 μm in (A), 10 μm in (B).

Acknowledgments

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