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Fig. S1 Patterning of the otic vesicle in sox3–pax8 morphants. Expression of dlx3b, otx1, pax5, and neurod in the otic vesicle of control embryos (A, E, I, Q), sox3 morphants (B, F, J, R), pax8 morphants (C, G, K, S) and sox3–pax8 double morphants (D, H, L, T) at 24 hpf. (M–P) brn3c:GFP expression at 30 hpf in a control embryo (M), sox3 morphant (N), pax8 morphant (O) and sox3–pax8 double morphant (P). Positions of utricular (u) and saccular (s) maculae are indicated. Images show dorsolateral views with anterior to the left (A–P); dorsal views with anterior to the top (Q–T).
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Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.