Fig. 2

Fig. 2 Aplexone does not affect arterial and venous fate. Untreated control embryos (A,A′,C,C′,E,E′) and embryos treated with 10 μM aplexone beginning at 10 hpf (B,B′,D,D′,F,F′) were fixed at 24 hpf. (A-F′) Arterial and venous fates were analyzed by in situ hybridization using ephrinB2a (A,A′,B,B′), ephB4 (C,C′,D,D′) and flt4 (E,E′,F,F′) probes. Arrowheads indicate the dorsal aorta. Embryos were analyzed at higher magnification in the trunk in A′-F′, with regions of interest indicated by black boxes in A-F. Arrows indicate the posterior cardinal vein.