ZFIN Image: Yoo et al., 2010, Fig. 1

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Figure Caption

Fig. 1 PI(3)Kγ Is Necessary for Directed Migration of Neutrophils In Vivo

(A) Sixty-five micromolar LY294002 treatment inhibits attraction of neutrophils to a laser wound in the caudal hematopoietic tissue (CHT). The lines indicate tracking of individual neutrophils over 30 min and the yellow thunder shows the position of the laser wound. Note that neutrophils migrate rapidly toward the wound in control (DMSO, but not after sLY294002 treatment (Movie S1). Scale bar = 50 μm.

(B) The number of neutrophils that reach the laser wound in the CHT within 30 min is quantified (seven movies for each condition, ^*p < 0.001, two-tailed unpaired t test). Note that LY294002 treatment inhibits attraction of neutrophils to the wound.

(C) Splice morpholino disturbs splicing of pi(3)kγ transcript. Mpo transcript indicates integrity of neutrophils in morphants.

(D) Knockdown of PI(3)Kγ inhibits directional migration of neutrophils to wounds (neutrophils are indicated with blue arrows in control, ^*p < 0.001, two-tailed unpaired t test). Data of (D) are representative of three separate experiments.

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Acknowledgments

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Reprinted from Developmental Cell, 18(2), Yoo, S.K., Deng, Q., Cavnar, P.J., Wu, Y.I., Hahn, K.M., and Huttenlocher, A., Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish, 226-236, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell