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Fig. S4 Efficient immunoprecipitation of Hdac1 protein from embryonic chromatin by anti-Hdac1 antibody. Crosslinked, sonicated chromatin was prepared from 12 hpf zebrafish embryos, then incubated with anti-Hdac1 antibody and negative control IgG. Immune complexes were precipitated with Protein G-agarose and both immunoprecipitated proteins and unbound proteins were analysed by SDS-PAGE and Western blotting with the anti-Hdac1 antibody, with an equivalent sample of input chromatin run alongside. All Hdac1 protein in the input sample was recovered in the anti-Hdac1 immunoprecipitate, whereas none of the Hdac1 protein was immunoprecipitated by the negative control IgG and it remained in the unbound fraction.