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Fig. 7 Increased her6 expression in the CNS of Hdac1ATG1 morphants and TSA-treated embryos during early neurogenesis. (A-F) Wild-type sibling (A, C, E) and Hdac1ATG1 morphant (B, D, F) embryos were analysed for expression of her6 at 12 hpf (A, B, C, D) and 18 hpf (E, F). (G-J) Embryos were cultured in the absence (G, I) or presence (H, J) of the HDAC inhibitor TSA (1 μM) from 10-14 hpf, then analysed for expression of her6. In dorsal and lateral views, anterior is to the left. Green arrowheads indicate caudal hindbrain, which exhibits increased her6 expression in Hdac1ATG1 morphants at 12 hpf and in TSA-treated embryos at 14 hpf; red arrows indicate optic vesicles, which exhibit increased her6 expression in Hdac1ATG1 morphants at 12 hpf and in TSA-treated embryos at 14 hpf; blue asterisks indicate hindbrain, which exhibits increased increased her6 expression in Hdac1ATG1 morphant embryos at 18 hpf.