Fig. 11

Fig. 11 Electrophoretic mobility shift assay showing specific binding of RAR/RXR with the two RAREs in the downstream region of hoxb1b. (A) The DIG-labeled oligonucleotides containing 3′-RARE1 and 3′-RARE3 (D1R and D4R, respectively) were incubated with zebrafish RAR/RXR in the absence or presence of 200-molar excess of competitors (D1R, D4R, and RfR), and run on a 5%-polyacrylamide gel. (B) DIG-labeled D1R and D4R probes were incubated with RAR/RXR in the absence or presence of 200-molar excess cognate oligonucleotides (D1R, D4R) or their mutated versions (D1m, D4m). (C) Sequences of the oligonucleotides used as probes or competitors. The RARE sequences are marked with underlines, and base substitutions are shown in lower case.