Fig. 10

Fig. 10 RARE sequences in the downstream region mediate the RA signal in the regulation of hoxb1b. (A) In F1-GFP and F4-GFP, F1 and F4 DNA were placed downstream to the egfp gene, respectively. The RAREs in these downstream subregions were deleted from the constructs by inverse PCR, giving rise to F1ΔR-GFP and F4ΔR-GFP. (B–I) Transient expression of GFP fluorescence in injected embryos at the specified stages. (D, E, H, I) The percentages of embryos showing expression in the posterior neural pate and the numbers of scored embryos (n) are shown at the bottom-right. The blastoderm margin, anterior boundary of reporter expression, and anterior end of the head are shown with dashed lines (B, F), open arrowheads (C, G), and open arrows (C, G), respectively. Scale, 200 μm.