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Fig. 5 Effect of longdaysin on the circadian period in zebrafish in vivo.
Zebrafish hemizygous for per3-luc were entrained in 12 h light/12 h dark cycles, and then luminescence rhythms were monitored in constant darkness in the presence of various concentrations of longdaysin. Representative luminescence rhythm of individual fish (n = 5 for each condition) was plotted after smoothing with 2.4 h moving average and baseline subtraction with fourth order polynomial curve (A). Period parameter was obtained by curve fitting and plotted against compound concentration (B) (n = 13, 5, 11, and 8 for 0, 1, 3, and 9 μM longdaysin, respectively). Representative pictures of zebrafish after 1 wk of treatment with longdaysin are shown (C). Scale bar, 1 mm.